SDS-PAGE Explained | Principle, Steps, and Applications | Protein Separation Technique
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SDS-PAGE Explained | Principle, Steps, and Applications | Protein Separation Technique
SDS Page understanding how scientists separate and analyze proteins in the lab? This video covers SDS-PAGE (Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis) — a core technique in molecular biology and biopharma. Learn: What SDS-PAGE is Principle and purpose Step-by-step gel preparation Visualization of protein bands Common ...
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SOLVED: Calculate the preparation of all samples to be run on your SDS-PAGE gel. You need to load 10 μg total protein in 3 μl. You need to prepare 30 μl of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below.Sample Protein Protein Water 5X Sample Total Sample Conc. Sample (μg) Buffer Volume (μl) (μl) (μl)CRUDE 30Desalt 30Peak Fraction 30Peak Fraction 30Note: You must complete this table before coming to class.
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