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9:41
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jove.com
Video: Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
13.0K Views. University of Zurich. With TRAP, we can investigate developmental processing at cellular resolution. We use cell-type-specific promoters to drive a tagged ribosomal sub-unit, and from there we can isolate polysome RNA. This protocol yields high quality RNA, but in very limited quantities, therefore, methods that are specialized for ...
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