How does UV-Vis spectrophotometry help with the understanding of failed reactions? Genomic analyses, such as next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR), require ...

To permanently access this Infographic for free, fill out the short form below. There are many different polymerase chain reaction (PCR) workflows, including end-point PCR, qualitative PCR (qPCR), and ...

The scope of real-time quantitative (qPCR) assays for gene-expression analysis in research applications and for sequence-specific nucleic acid detection for diagnostic purposes continues to grow.

Reverse transcription quantitative real-time PCR (RT-qPCR) is the most commonly used method for the quantification of mRNA expression levels. Generally, the starting material for RTqPCR is purified ...

Real-time quantitative PCR (qPCR) is a powerful tool to detect and quantify nucleic acids. By incorporating fluorescently labeled probes or fluorescent double-stranded DNA (dsDNA)-binding dyes into ...

When working in a lab, launching a quantitative PCR (qPCR) experiment demands a comprehensive understanding of complicated protocols, principles, and methodologies. Analyzing the large amounts of ...

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting ...

From the start of the COVID-19 pandemic, diagnostic testing has been highlighted as a key part of global measures to contain the spread of SARS-CoV-2. In a media briefing on March 16, 2020, Dr Tedros ...

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...