Accurate assembly of rRNA-depleted (total) RNA sequencing (RNA-seq) remains challenging because existing methods often conflate incomplete, nascent RNA with fully processed mature isoforms, leading to ...

DNA-based methods dominate molecular cancer diagnostics but struggle to detect gene fusions and assess splice site consequences. RNA sequencing enables sensitive fusion detection and direct assessment ...

Alternative polyadenylation (APA) and alternative splicing (AS) are essential post-transcriptional mechanisms that enhance transcriptome diversity and regulate gene expression across various ...

Researchers from Children's Hospital of Philadelphia (CHOP) developed a new RNA sequencing strategy that can reveal how genetic variants disrupt gene function and improve the diagnosis of rare ...

The high complexity and diversity of the eukaryotic transcriptome poses significant challenges for the efficient detection of specific transcripts. Conventional targeted RNA-seq methods often require ...

A new single-cell technology is giving scientists their clearest view yet of immune cell behavior—capturing not just genetic intent, but real-time activity. By measuring RNA and proteins ...

Years of experiments and databases filled with RNA-seq results belie the simple reality that, until fairly recently, it was impossible to analyze RNA directly. The RNA-seq studies performed with ...

While bulk sequencing quickly became the preferred method, scRNA-seq has increased in popularity over the last decade. In 2015, just 6 % of RNA-seq papers used single-cell methodologies. By 2025, the ...

Morgan Hiebert (left) studies drug-resistant tuberculosis within the National Reference Centre for Mycobacteriology (Winnipeg, Manitoba) led by Hafid Soualhine, situated in the National Microbiology ...

Evaluation of scCNV inference methods using a clinical small cell lung cancer (SCLC) dataset. (A) Sensitivity and specificity of the four scCNV inference methods applied to primary and relapse SCLC ...

A comprehensive review article titled “Bioinformatics perspectives on transcriptomics: A comprehensive review of bulk and single-cell RNA sequencing analyses,” published in Quantitative Biology, ...

Bacteria aren’t just mindless microbes. Research by scientists at the Hebrew University of Jerusalem has revealed that single bacterial cells can carry a “memory” of their past environments—passing it ...