The immune system comprises distinct functionally specialized cell populations, which can be characterized in depth by mass cytometry protein profiling. Unfortunately, the low-throughput nature of ...

Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data.

Much of what biologists know about the human immune system has been — quite literally — illuminated by flow cytometry. For more than 70 years, this technology has evolved to become one of immunology’s ...

Many common flow cytometry mistakes share a common feature—simply following procedures instead of cultivating conceptual ...

The interaction between cells in the central nervous system and immune cells are key in neuroinflammation, and play a role in the survival of neurons during neuropathology. Flow cytometry is a ...

In this case study, researchers at the Weizmann Institute of Science in Israel developed and optimized an imaging flow cytometry (IFC) protocol to provide a high-throughput, quantitative and highly ...

If you work in cell biology or immunology, chances are that you're familiar with flow cytometry. This technique measures and analyzes biological cells by suspending them in a stream of fluid and ...

Human umbilical vein endothelial cells (HUVECs) were seeded at 1 × 10 4 cells per cm 2 in six-well plates. Treatment with TNF-α (2.5, 5 or 10 ng/ml) or lipopolysaccharide (0.5, 1 or 5 μg/ml) treatment ...